alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Assay Kit (Colorimetric)
Referentie MBS9719206-96
Formaat : 96Tests
Merk : MyBiosource
Alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9719206PROMOTIONAL PRICING: 5% OFF web . Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed
Principle of the Assay: alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) is widely present in the mitochondria of animals, plants, microorganisms and in-vitro cultured cells. It is one of the key regulation enzymes of Kreb's Cycle, catalyzing the oxidation and… More details
Product Overview
Online Datasheet in PDFProduct Name
alpha-Ketoglutarate Dehydrogenase (alpha-KGDH), Assay Kit
Full Product Name
alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Assay Kit (Colorimetric)
Product Gene Name
alpha-KGDH assay kit
Samples
Animal and Plant Tissues, Cells
Preparation and Storage
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Usage Notes
deltaA of Test Tube should be between 0.01-0.3, the sample should be diluted further if deltaA of Test Tube >0.3.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract alpha-KGDH from fresh samples in order to ensure the enzyme activity.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract alpha-KGDH from fresh samples in order to ensure the enzyme activity.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Product Categories/Family for alpha-KGDH assay kit
Biochemical Assay Kit; Cell Metabolism; Cytology
Other Notes
Small volumes of alpha-KGDH assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for alpha-KGDH purchase
MBS9719206 is an assay kit targeting alpha-KGDH. Also known as alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Assay Kit (Colorimetric).