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> Annonacin [111035-65-5]

Annonacin [111035-65-5]

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Referentie HY-N2877-1mg

Formaat : 1mg

Merk : MedChemExpress


Description

Annonacin is an acetylgenin that is toxic by inhibiting the pathway of the mitochondrial complex. Annonacin increases tau phosphorylation in R406W+/+ mice. Annonacin acts as an inhibitor of the sodium/potassium and sarcoplasmic reticulum (SERCA) ATPase pumps. Annonacin has significant killing effect on ovarian cancer cell, cervical cancer cell, breast cancer cell, bladder cancer cell and skin cancer cell. Annonacin induces apoptosis through Bax and Caspase-3-related pathways[1][2][3][4].

In Vitro

Annonacin (1-10 μg/mL; 0-72 h) induces the death of a variety of cancer cell lines, and blocks cancer cells in G1 phase [2].
Annonacin 10 μg/mL; 0-24 h) induces apoptosis of T24 cells and enhance the expression of pro-apoptosis-related genes [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Annonacin Related Antibodies

Cell Viability Assay[2]

Cell Line: T24 cells
Concentration: 1 μg/mL
Incubation Time: 0, 24, 48 and 72 h
Result: Significantly reduced cell viability. Compared with untreated controls, only 56.0%, 34.2% and 35.3% of T24 cells were alive at 24, 48 and 72 hours, respectively.

Western Blot Analysis[2]

Cell Line: T24 cells
Concentration: 10 μg/mL
Incubation Time: 1, 4, 8, 12 and 24 h
Result: Increased the levels of Bax and Bad.
Inhibited the levels of procaspase-3.
In Vivo

Annonacin (0-9 mg/kg; Subcutaneous osmotic pump; 3 days) increases tau phosphorylation in R406W+/+ mice [3].
Annonacin (85 nM/100 μL; Topical application; Twice a week for 22 weeks) has anti-cancer effect in mouse model of skin tumor [4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: R406W+/+ transgenic mice[3]
Dosage: 0, 6 and 9 mg/kg/day
Administration: Subcutaneous osmotic pump; 3 days
Result: Increased the number of neurons with phosphorylated tau in the somatodendritic compartment in several brain areas.
Increased the levels of phosphorylated tau protein.
Increased in total tau protein without increase in tau mRNA, but reduced proteasomal proteolytic activity.
Increased the levels of different tau kinases.
Significantly increased the ratio of p25/p35.
Animal Model: DMBA (HY-W011845)/TPA treated female ICR mice aged 6-7 weeks old[4]
Dosage: 85 nM/100 uL
Administration: Topical application; Twice a week for 22 weeks
Result: Significantly increased the tumor latency period and reduced the tumor incidence, tumor burden and tumor volume, respectively.
Suppressed tumorigenesis manifested by significant reduction of hyperkeratosis, dermal papillae and number of keratin pearls on skin tissues.
Showed no toxicity to the liver and kidneys.
Masse moléculaire

596.88

Formule

C35H64O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1O[C@@H](C)C=C1C[C@H](O)CCCCC[C@@H](O)CCCC[C@@H](O)[C@@H]2O[C@@H]([C@H](O)CCCCCCCCCCCC)CC2
Structure Classification
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (167.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6754 mL 8.3769 mL 16.7538 mL
5 mM 0.3351 mL 1.6754 mL 3.3508 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Pureté et documentation
Références

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