Syrian Hamster monoclonal antibody PE/Cy5 labled to CD28

Referentie CFG8787-50

Formaat : 50Assays

Merk : Cohesion Biosciences

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Product NameAnti-CD28 Antibody-PE/Cy5 labled
Cat No: CFG8787
Source: Syrian Hamster
Reactivity: M
Applications: IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Syrian Hamster monoclonal antibody PE/Cy5 labled to CD28
Immunogen: C57BL/6 mouse T-cell lymphoma EL-4
Purification: The antibody was purified by affinity chromatography.
Clonality: Monoclonal (clone: 37.51)
Conjugation: PE/Cy5
Form: Syrian Hamster IgG. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution: 10 μl / assay
Gene Symbol: CD28
Alternative Names: T-cell-specific surface glycoprotein CD28; TP44; CD28
Entrez Gene (Mouse) :
SwissProt (Mouse) :
Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.