Anti-DKK1 Antibody (8S801)
Referentie NB-64-116500-100uL
Formaat : 100uL
Merk : Neo Biotech
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Anti-DKK1 Antibody (8S801)
(Synonyms: SK, hDkk-1, hDkk1, Dkk-1, Dkk1, Dickkopf-related 1, Dickkopf-1) Copy Product InfoSynonyms: SK, hDkk-1, hDkk1, Dkk-1, Dkk1, Dickkopf-related 1, Dickkopf-1
Catalog No. TMAC-01151 Copy Product Info
Anti-DKK1 Antibody (8S801) is a Rabbit antibody targeting DKK1. Anti-DKK1 Antibody (8S801) can be used in FCM,ICC/IF,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-DKK1 Antibody (8S801) is a Rabbit antibody targeting DKK1. Anti-DKK1 Antibody (8S801) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | SK, hDkk-1, hDkk1, Dkk-1, Dkk1, Dickkopf-related 1, Dickkopf-1 |
| Ig Type | IgG |
| Clone | 8S801 |
| Reactivity | Human,Rat |
| Verified Activity | 1. Western blot analysis of DKK1 on MCF-7 cells lysates using anti-DKK1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DKK1 antibody. Counter stained with hematoxylin. 3. ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining DKK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with DKK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.  , , , , , |
| Application | FCM ICC/IF IHC WB |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID | O94907 |
| Molecular Weight | Theoretical: 38 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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