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Anti-Guinea Pig IgG (H&L) (Biotin Conjugated) Pre-Adsorbed Secondary Antibody

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Formaat : 1mg

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Donkey Polyclonal, Biotin

Western Blot of Donkey anti-Guinea Pig IgG (Pre-Adsorbed) Biotin Conjugated Secondary Antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Biotin conjugated donkey anti-guinea pig IgG 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.

Description	Anti-GUINEA PIG IgG (H&L) (DONKEY) Antibody Biotin Conjugated (Min X Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)
Host	Donkey
Conjugate	Biotin
Target Species	Guinea Pig
Reactivity	Guinea Pig
Clonality	Polyclonal
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, E, IC
Application Note	ELISA 1:10,000-1:50,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000
Physical State	Lyophilized
Host Isotype	IgG
Target Isotype	IgG (H&L)
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen	Guinea Pig IgG whole molecule
Reconstitution Volume	1.0 mL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide

Shipping Condition	Ambient
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas