Anti-Human CD11c [Clone 3.9] – Biotin
Referentie C146-50
Formaat : 50ug
Merk : Leinco Technologies
AntiHuman CD11c [Clone 3.9] — Biotin
AntiHuman CD11c [Clone 3.9] — Biotin
Product No.: C146
Clone 3.9 Target CD11c Formats AvailableView All Product Type Monoclonal Antibody Alternate Names gp 150/95 Isotype IgG1 Applications FC |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Product Concentration 0.5 mg/ml Formulation This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This biotinylated antibody is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828523 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 3.9 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.> Additional Reported Applications For Relevant Conjugates ? IHC (Frozen) FA For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 3.9 recognizes the αchain (CD11c) of the CD11c/CD18 complex. It is specific for the I domain of CD11c. Clone 3.9 binds the activated form of CD11c and partially blocks the binding of CD11c with ICAM4. Background LFA1α (CD11a) and CD18 are the Integrin alphaL and beta2 chains respectively that combine to form LFA1, a glycoprotein and a member of the Integrin family. Integrin alphaL/beta2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA1 participates in the immunological synapses between CD8+ T lymphocytes and antigenpresenting cells. The absence of LFA1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyteendothelial cell interaction, cytotoxic Tcell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. Ligand/Receptor CD54, fibrinogen, iC3b, ICAM1, ICAM4 PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Innate Immunity . Neuroscience . Neuroscience Cell Markers References & Citations1. Leukocyte Typing V (1995) Oxford University Press 2. Leukocyte Typing IV (1989) Oxford University Press 3. Leukocyte Typing III (1987) Oxford University Press 4. Hogg, N. et al. (1986) Eur. J. Immunol. 16:240 5. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117 |