Anti-Malondialdehyde Mouse Monoclonal Antibody (56615)
Referentie 56615-100
Formaat : 100ug
Merk : Leinco Technologies
AntiMalondialdehyde Mouse Monoclonal Antibody (56615)
AntiMalondialdehyde Mouse Monoclonal Antibody (56615)
Product No.: 56615
Clone 6H6 Target Malondialdehyde Formats AvailableView All Product Type Monoclonal Alternate Names Malondialdehyde, MDA, Malondialdehyde (MDA), Malonic aldehyde Propanedial, 1,3Propanedial, Malonaldehyde Isotype Mouse IgG1 Applications ELISA , FACS , ICC , IF , WB , FCM |
Data
Flow Cytometry analysis using Mouse AntiMalondialdehyde Monoclonal Antibody, Clone 6H6 . Tissue: Neuroblastoma cells (SHSY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse AntiMalondialdehyde Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat AntiMouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Western Blot analysis of MalondialdehydeBSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse AntiMalondialdehyde Monoclonal Antibody, Clone 6H6 . Lane 1: Molecular Weight Ladder (MW). Lane 2: MalondialdehydeBSA (0.5 µg). Lane 3: MalondialdehydeBSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg) . Block: 5% Skim Milk in TBST. Primary Antibody: Mouse AntiMalondialdehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat AntiMouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/Immunofluorescence analysis using Mouse AntiMalondialdehyde Monoclonal Antibody, Clone 6H6 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse AntiMalondialdehyde Monoclonal Antibody at 1:50 for 3060 min at RT. Secondary Antibody: Goat AntiMouse Alexa Fluor 488 at 1:1500 for 3060 min at RT. Counterstain: Phalloidin Alexa Fluor 633 FActin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 3060 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) – Untreated. (B,D,F,H) – Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 FActin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.Antibody DetailsProduct DetailsReactivity Species Species Independent Host Species Mouse Immunogen Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH). Product Concentration 1 mg/mL Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide State of Matter Liquid Product Preparation Protein G Purified Storage and Handling This antibody is stable for at least one (1) year at 20°C. Avoid multiple freezethaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 28°C Applications and Recommended Usage? Quality Tested by Leinco WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); Optimal dilutions for assays should be determined by the user. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not crossreact with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4Hydroxy2hexenal, 4Hydroxy nonenal, or Methylglyoxal modified proteins. Background Malondialdehyde (MDA) is the biomarker in greatest diagnostic use, due to its molecular stability. This threecarbon, lowmolecular weight aldehyde has a strong affinity for amino acids, which results in adduct formation to both free amino acids and proteins. Increased MDA levels have been found at correlating levels in breast cancer, and lung cancer patients. Other diseased states with elevated MDA levels include diabetes and Alzheimer’s disease. Multiple laboratory techniques exist for quantification of MDA levels, including the thiobarbituric acid reactive substances (TBARS) assay. In addition to use as a biomarker, MDA has been shown to have mutagenic effects on tissues themselves as adduct formation can result in DNA crosslinking. Antigen DetailsResearch Area Cancer . Neuroscience . Alzheimer's Disease . Lipid peroxidation . Neurodegeneration . Oxidative Stress References & Citations |