General Information
| Short Description | Rabbit polyclonal NANOG (1-105) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen Region | 1-105 |
| Immunogen Sequence | MSVDPACPQSLPCFEASDCK ESSPMPVICGPEENYPSLQM SSAEMPHTETVSPLPSSMDL LIQDSPDSSTSPKGKQPTSA EKSVAKKEDKVPVKKQKTRT VFSST |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Nanog (NP_079141.2). |
Additional Info
| Tissue Specificity | Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Protein Name | Homeobox Protein NanogHomeobox Transcription Factor NanogHnanog |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nanog antibodyAnti-Homeobox Transcription Factor Nanog antibodyAnti-Hnanog antibodyAnti-NANOG antibody |
Information sourced from Uniprot.org