Mouse anti-Human/Mouse/Rat/Monkey/Pig/Chicken/Zebrafish/Drosophila melanogaster/Yeast PCNA Antibody [Sodium Azide Free]
Anti-PCNA Antibody, Sodium Azide Free
Anti-PCNA Antibody, Sodium Azide Free
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Product Description
Specifications
| Size | 100 µg |
| Species | Human, Mouse, Rat |
| Host Species | Mouse |
| Accession Number | P12004 |
| Gene Id | 5111 |
| Gene Symbols | PCNA |
| Protein Name / Synonyms | Proliferating cell nuclear antigen (PCNA) (Cyclin) |
| Immunogen | Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody. |
| Isotype | Kappa, IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Predominantly nuclear, some cytoplasmic |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
This antibody recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.
Storage
Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.