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> Anti-Phospho-AKT1 (Ser473) Antibody (6A70)

Anti-Phospho-AKT1 (Ser473) Antibody (6A70)

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Referentie NB-64-118534-50uL

Formaat : 50uL

Merk : Neo Biotech


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Anti-Phospho-AKT1 (Ser473) Antibody (6A70)

(Synonyms: p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)) Copy Product Info

Synonyms: p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)

Catalog No. TMAC-03188 Copy Product Info
Anti-Phospho-AKT1 (Ser473) Antibody (6A70) is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Antibody (6A70) can be used in ICC/IF,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Phospho-AKT1 (Ser473) Antibody (6A70) is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Antibody (6A70) can be used in ICC/IF,IHC,WB.
Synonymsp-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)
Ig Type
IgG
Clone
6A70
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of Phospho-Akt1(Ser473) on different lysates using anti-Phospho-Akt1 (Ser473) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 treated with PDGF, Lane 2: NIH/3T3 untreated.
2. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Phospho-Akt1 (Ser473) antibody. Counter stained with hematoxylin.
3. ICC staining Phospho-Akt1 (Ser473) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Anti-Phospho-AKT1 (Ser473) Antibody (6A70),Anti-Phospho-AKT1 (Ser473) Antibody (6A70),Anti-Phospho-AKT1 (Ser473) Antibody (6A70)
Application
ICC/IF
IHC
WB
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
A synthesized phosphopeptide: human Akt1 around the phosphorylation site of Ser473
Antigen Species
Human
Uniprot ID
P31749
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

pAKT1 (Ser473)pAKT1 (S473)AKT1 (pSer473)AKT1 (pS473)AKT1 (phospho-Ser473)AKT1 (phospho-S473)
Related Tags: Anti-Phospho-AKT1 (Ser473) Antibody (6A70) molecular weight