Anti-SARS-CoV-2 Nucleocapsid (N) (Clone NP1-F3) - HRP
Referentie LT7052-50
Formaat : 50ug
Merk : Leinco Technologies
AntiSARSCoV2 Nucleocapsid (N) (Clone NP1F3) – HRP
AntiSARSCoV2 Nucleocapsid (N) (Clone NP1F3) – HRP
Product No.: LT7052
Product No.LT7052 Clone NP1F3 Target SARSCoV2 Nucleocapsid (N) Product Type Recombinant Monoclonal Antibody Alternate Names COV2NP1F3, SARSCoV2 Nucleocapsid, SARSCoV2 Nucleoprotein, Protein N, SARSCoV N Protein Isotype Human IgG1 Applications ELISA |
Antibody DetailsProduct DetailsReactive Species SARSCoV2 ⋅ Virus Expression Host HEK293 Cells Immunogen SARSCoV2 Nucleocapsid (N) Protein Product Concentration 0.5 mg/ml Formulation This HRPconjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase) Storage and Handling This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Ships Overnight on Blue Ice RRIDAB_2894001 Applications and Recommended Usage? Quality Tested by Leinco ELISA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity AntiSARSCoV2 Nucleocapsid, clone NP1F3, specifically targets an epitope on the SARSCoV2 nucleocapsid protein. Furthermore, it is reported to bind to the RNA binding domain of the N protein. Background Coronavirus disease 2019 (COVID19) is caused by the Coronaviridae family virus severe acute respiratory syndrome coronavirus 2 (SARSCoV2)1. SARSCoV2 has four structural proteins encoded by its singlestranded, positivesense RNA genome: the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins2. The N protein is a highly conserved 46 kDa protein that shares 90% homology with SARSCoV3. The N protein has an Nterminal (NTD) and Cterminal domain (CTD), which bind to RNA and selfoligomerize, respectively4,5, forming a helical nucleocapsid structure within the viral envelope6. Other functions of the N protein included viral transcription, replication, and modulating cell signaling pathways7,8. The N protein is abundantly expressed during infection, and antibodies3,9 and memory T cells10,11 targeting the N protein have been identified in convalescent sera. Therefore, the N protein is a target in some vaccines and diagnostic assays12. The N protein also has therapeutic potential, as it evades RNAimediated antiviral responses13. Antigen Distribution The nucleocapsid protein is expressed in the internal nucleocapsid of SARSCoV2. NCBI Gene Bank ID Research Area COVID19 . Infectious Disease . Seasonal and Respiratory Infections . Viral . IVD Raw Material References & Citations1. Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270–273. 2020. 2. Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020. 3. Guo L., Ren L., Yang S., et al. Clinical Infectious Diseases: an Official Publication of the Infectious Diseases Society of America. 2020. 4. Kang S, Yang M, Hong Z, et al. Acta Pharm Sin B. 10.1016/j.apsb.2020.04.009. 2020. 5. Chang CK, Sue SC, Yu TH, et al. J Biomed Sci. 13(1):5972. 2006. 6. Hsieh PK, Chang SC, Huang CC, et al. J Virol. 79(22):1384813855. 2005. 7. Surjit M, Lal SK. Infect Genet Evol. 8(4):397405. 2008. 8. Hurst KR, Ye R, Goebel SJ, Jayaraman P, Masters PS. J Virol. 84(19):1027610288. 2010. 9. To K.K., Tsang O.T., Leung W.S., et al. Lancet Infect. Dis. 2020. 10. Grifoni A., Weiskopf D., Ramirez S.I., et al. Cell. 2020. 11. Ni L, Ye F, Cheng ML, et al. Immunity. 52(6):971977.e3. 2020. 12. Liu L, Liu W, Zheng Y, et al. Microbes Infect. 22(45):206211. 2020. 13. Mu J, Xu J, Zhang L, et al. Sci China Life Sci. 14. 2020. |