| Short Description : | Rabbit polyclonal STX1A (1-288) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | |
| Gene ID: | |
| Uniprot ID: | |
| Immunogen Region: | 1-288 |
| Immunogen Sequence: | MKDRTQELRTAKDSDDDDDV AVTVDRDRFMDEFFEQVEEI RGFIDKIAENVEEVKRKHSA ILASPNPDEKTKEELEELMS DIKKTANKVRSKLKSIEQSI EQEEGLNRSSADLRIRKTQH STLSRKFVEVMSEYNATQSD YRERCKGRIQRQLEITGRTT TSEELEDMLESGNPAIFASG IIMDSSISKQALSEIETRHS EIIKLENSIRELHDMFMDMA MLVESQGEMIDRIEYNVEH |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human STX1A (NP_004594.1). |
| Tissue Specificity | Isoform 1: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2: Expressed in heart, liver, fat, skeletal muscle, kidney and brain. |
| Post Translational Modifications | Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1. Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis. |
| Function | Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1). |
| Protein Name | Syntaxin-1aNeuron-Specific Antigen Hpc-1 |
| Database Links | |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneSingle-Pass Type Iv Membrane ProteinSynapseSynaptosomeCell MembraneColocalizes With Kcnb1 At The Cell MembraneIsoform 2: Secreted |
| Alternative Antibody Names | Anti-Syntaxin-1a antibodyAnti-Neuron-Specific Antigen Hpc-1 antibodyAnti-STX1A antibodyAnti-STX1 antibody |
Information sourced from Uniprot.org