- More Files
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Trastuzumab monoclonal antibody, clone 15H2 (Cat # MAB23091) with Trastuzumab. Coating antigen: Trastuzumab, 1 ug/mL. Anti-Trastuzumab monoclonal antibody, clone 15H2 dilution start from 1000 ng/mL. EC50 = 2.996 ng/mL. While the antibody does not recognize the human IgG (data not shown).
Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Trastuzumab monoclonal antibody, clone 11C4 (Cat # MAB23090) and Anti-Trastuzumab monoclonal antibody, clone 15H2 (Cat # MAB23091) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Trastuzumab monoclonal antibody, clone 15H2 was labeled with Biotin. The sensitivity of detecting Trastuzumab is up to 61 pg/mL.
- Specification
Product Description
Mouse monoclonal antibody raised against Trastuzumab.
Target gene is HER2/neu.Immunogen
Trastuzumab.
Host
Mouse
Specificity
The product is specific for Trastuzumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody : Anti-Trastuzumab monoclonal antibody, clone 11C4 (Cat # MAB23090).
Form
Lyophilized
Preparation Method
The antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Trastuzumab.
Purification
Protein A purification
Isotype
IgG1, kappa
Recommend Usage
ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
Non-inhibitory
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Trastuzumab monoclonal antibody, clone 15H2 (Cat # MAB23091) with Trastuzumab. Coating antigen: Trastuzumab, 1 ug/mL. Anti-Trastuzumab monoclonal antibody, clone 15H2 dilution start from 1000 ng/mL. EC50 = 2.996 ng/mL. While the antibody does not recognize the human IgG (data not shown).Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Trastuzumab monoclonal antibody, clone 11C4 (Cat # MAB23090) and Anti-Trastuzumab monoclonal antibody, clone 15H2 (Cat # MAB23091) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Trastuzumab monoclonal antibody, clone 15H2 was labeled with Biotin. The sensitivity of detecting Trastuzumab is up to 61 pg/mL. - Gene Info — ERBB2
Entrez GeneID
2064Gene Name
ERBB2
Gene Alias
CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
Other Designations
c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
- Interactome
- Pathway
- Disease