31-1062-00 | Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody

Product Details
Product Name	Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175
Product Description	Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
Catalog No.	31-1062-00
Clone Name	RM175
Product Data Sheet	Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175
Specificity	RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
Immunogen	A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
Application	Chromatin IP (ChIP) Western Blot (WB) Immunohistochemistry (IHC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 µg
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ChIP: 1 µg - 5 µg; Western Blot: 1 µg/mL – 2 µg/mL; IHC: 0.5 µg/mL – 2 µg/mL; ELISA: 0.5 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL.
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Product Images
	RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 µg/mL, showed a band of histone H3 trimethyl- ated at Lysine 27 (K27me3) in HeLa cells.
	Immunohistochemistry staining of HepG2 cells using Anti-Trimethyl-Histone H3 (Lys27) antibody, Clone RM175.
	Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using Anti-Trimethyl- Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175.
	Immunohistochemical staining of formalin fixed and paraffin embedded human glioblastoma tissue section using Anti-Trimethyl- Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175.
	Immunohistochemical staining of formalin fixed and paraffin embedded human tumor tissue section using Anti-Trimethyl- Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175, from user review.
References for Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27) [RM175]	Andrea J Kriz et al. Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation Mol Cell (IF: 17.97; Q1) . 2021 May 6;81(9):1970-1987. ChIP; Read more (PMID: 33725485) Chia-Chi Tsai et al. Overexpression of Histone H3 Lysine 27 Trimethylation Is Associated with Aggressiveness and Dedifferentiation of Thyroid Cancer Endocr Pathol 2019 Dec;30(4):305-311. Human IHC Read more (PMID: 31396854) Chen-Yu Wang et al. PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments Nat Commun (IF: 14.92; Q1). 2019 Jul 3;10(1):2950. ICC/IF ; Read more (PMID: 31270318) Peitao Lü et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. Nat Plants (IF: 13.26; Q1). 2018 Oct;4(10):784-791 ChIP; Read more (PMID: 30250279) Chen-Yu Wang et al. SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X Cell (IF: 41.58; Q1). 2018 Jul 12;174(2):406-421. ICC/IF ; Read more (PMID: 29887375)

Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175]

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