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> Aristolochic acid A (sodium) [10190-99-5]

Aristolochic acid A (sodium) [10190-99-5]

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Referentie HY-N0510A-10mg

Formaat : 10mg

Merk : MedChemExpress


Description

Aristolochic acid A (Aristolochic acid I) sodium is the main component of plant extract Aristolochic acids, which are found in various herbal plants of genus Aristolochia and Asarum. Aristolochic acid A sodium significantly reduces both activator protein 1 (AP-1) and NF-κB activities. Aristolochic acid A sodium reduces BLCAP gene expression in human cell lines[1].

In Vitro

Aristolochic acid A (150, 200 μM, 24 hours) sodium inhibits the cell viabilities of kidney cells HEK293 and HK-2[1].
Aristolochic acid A (100, 200 μM, 24 hours) sodium causes a concentration-dependent decrease in bladder cancer-associated protein (BLCAP) mRNA levels in kidney cells (HEK 293 and HK-2) , and bladder cancer cell line (HT-1376)[1].
Aristolochic acid A (100, 200 μM, 24 hours) sodium weakens the BLCAP protein signals in a dose-dependent manner in both HEK293 and HT-1376 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Aristolochic acid A sodium Related Antibodies

Cell Viability Assay[1]

Cell Line: Kidney cells (HEK 293 and HK-2)
Concentration: 50, 100, 150, 200 μM
Incubation Time: 24 hours
Result: The cell viabilities of HEK293 and HK-2 were lower than that of vehicle-treated cultures until 150 μM and 200 μM.

RT-PCR[1]

Cell Line: Kidney cells (HEK 293 and HK-2), and bladder cancer cell line (HT-1376)
Concentration: 100, 200 μM
Incubation Time: 24 hours
Result: Down regulated the levels of BLCAP mRNA.

Western Blot Analysis[1]

Cell Line: HEK293 and HT-1376 cells
Concentration: 100, 200 μM
Incubation Time: 24 hours
Result: Reduced the BLCAP protein expression in a dose-dependent manner.
In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Aristolochic acid A can be used to induce nephrotoxicity models. Pharmacokinetic studies show that in male C57BL/6 mice, the concentration of Aristolochic acid A in the kidneys is higher than in the liver 30 minutes after intraperitoneal administration (10 mg/kg). The plasma concentration of Aristolochic acid A also peaks approximately 30 minutes after administration[2].

Induction of Nephrotoxicity [2][3]
Background
The pathogenesis of Aristolochic acid Anephropathy remains unclear. Research shows that Aristolochic acid A mainly damages renal tubular epithelial cells and renal tules, leading to interstitial fibrosis, thereby producing nephrotoxicity.
Specific Modeling Methods
Mice: C57BL/6J • 8 weeks of age
Administration: 10 mg/kg • i.p. • 3-5 days[3]
Modeling Indicators
Pathology changs: H&E stain shows renal tissue structure is disordered, glomerular edema, pyknosis, and proximal tubule epithelial cell shedding
Biochemistry changes: Scr and blood urea nitrogen (BUN) contents increases

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Masse moléculaire

363.25

Formule

C17H10NNaO7
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(O[Na])C1=CC2=C(OCO2)C3=C4C(C(OC)=CC=C4)=CC([N+]([O-])=O)=C31
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvant et solubilité
In Vitro: 

H2O : 10 mg/mL (27.53 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7529 mL 13.7646 mL 27.5293 mL
5 mM 0.5506 mL 2.7529 mL 5.5058 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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