Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Atglistatin [1469924-27-3]

Afdrukken

Referentie NB-64-08757-1mL

Formaat : 1mLx10mM(inDMSO)

Merk : Neo Biotech

Meer informatie aanvragen

Remove All

Atglistatin

Copy Product Info

Synonyms:

Catalog No. T1875 Copy Product Info

Purity: 99.01%

Atglistatin is a highly effective, specific and competitive ATGL inhibitor with an IC50 of ~0.7 μM for inhibition of lipolysis in vitro, and no toxicity when the concentration up to 50 μM.

Cas No. 1469924-27-3

For research use only—not for human use. No sales to individuals. Use as intended only.

Select Batch

Purity:99.01%

Color:White to Pink

Product Introduction

Bioactivity

Description	Atglistatin is a highly effective, specific and competitive ATGL inhibitor with an IC50 of ~0.7 μM for inhibition of lipolysis in vitro, and no toxicity when the concentration up to 50 μM.
Targets & IC50	ATGL:0.7 μM
In vitro	Atglistatin inhibits ATGL, consequently suppressing lipid breakdown in both cellular and organ cultures without exhibiting cytotoxic effects at concentrations up to 50 µM.
In vivo	Atglistatin, administered orally (p.o.), reduces fatty acids (FA) and glycerol by up to 50% and 62% respectively, in a dose-dependent manner, and also significantly reduces triglyceride (TG) levels in the plasma by 43%. When administered via intraperitoneal injection (i.p.), Atglistatin inhibits lipolysis in a dose- and time-dependent manner. Moreover, Atglistatin exhibits significant differences in tissue distribution, primarily accumulating in the liver and adipose tissue.
Kinase Assay	Determination of lipase activity: For determination of lipase activity, lysates are incubated with a substrate containing radiolabeled [9,10-3H(N)]-triolein. Subsequently, FA are extracted and quantitated by liquid scintilation counting. Data are presented as mean S.D. of triplicate determinations and representative for at least three independent experiments.
Cell Research	For MTT-based in vitro viability assays, cells are seeded in 96-well plates and cultured under standard conditions for 24 h. The next day, cells are pretreated with different concentrations of Atglistatin dissolved in DMSO or Cisplatin dissolved in DMF as positive control for 2 h. Medium is replaced by an identical fresh medium and incubated again for the indicated time points. Thereafter, cells are incubated for 3 h with 100 μL Thiazolyl Blue Tetrazolium Bromide (MTT). The resulting violet formazan crystals are dissolved by adding 100 μL of MTT solubilization solution (0.1% NP-40, 4 mM HCl and anhydrous isopropanol). After complete dissolution of the formazan product, absorbance is measured at 595 nm using 690 nm as reference wavelength.(Only for Reference)

Chemical Properties

Molecular Weight	283.37
Formula	C₁₇H₂₁N₃O
Cas No.	1469924-27-3
Smiles	CN(C)C(=O)Nc1cccc(c1)-c1ccc(cc1)N(C)C
Relative Density.	1.135 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 100 mg/mL (352.9 mM), Sonication is recommended.

Ethanol: 4 mg/mL (14.12 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (14.12 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.5290 mL	17.6448 mL	35.2896 mL	176.4478 mL
5 mM	0.7058 mL	3.5290 mL	7.0579 mL	35.2896 mL
10 mM	0.3529 mL	1.7645 mL	3.5290 mL	17.6448 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1764 mL	0.8822 mL	1.7645 mL	8.8224 mL
50 mM	0.0706 mL	0.3529 mL	0.7058 mL	3.5290 mL
100 mM	0.0353 mL	0.1764 mL	0.3529 mL	1.7645 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

InhibitorinhibitAtglistatinATGLAdipose triglyceride lipase

Related Tags: Atglistatin chemical structure | Atglistatin in vivo | Atglistatin in vitro | Atglistatin formula | Atglistatin molecular weight