beta-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit
Referentie MBS2576272-96
Formaat : 96Tests
Merk : MyBiosource
Beta-Secretase 1 (BACE 1) (BACE1) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2576272MBS2576272 is an assay kit targeting BACE1. Also known as beta-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit. More details
Product Overview
Product Name
beta-Secretase 1 (BACE 1) (BACE1), Assay Kit
Full Product Name
beta-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit
Product Gene Name
BACE1 assay kit
Product Synonym Names
BACE 1
Samples
serum, plasma, tissue, cell
Detection Range
0.003-4.75 U/L
Sensitivity
0.003 U/L
Preparation and Storage
This product can be stored at -20 degree C for 12 months with shading light.
Shipping: Ice bag
Shipping: Ice bag
Asssay Type
Enzyme Activity
Detection Type
Fluorescence method
Detection Instrument
Fluorescence Microplate Reader
Precision
Inter CV: 4.7-10.4
Intra CV: 2.2-3.2
Intra CV: 2.2-3.2
Research Area
Amino Acids and Proteins
Test Principle
beta-Secretase 1 is an aspartic protease, the full name is beta-site amyloid precursor protein cleavage enzyme 1(BACE 1). BACE 1 cleaves amyloid precursor protein (APP) to produce amyloid-beta peptide (Abeta) of 40 or 42 amino acids. The deposition of Abeta in plaques and vessel walls in the brains of patients with Alzheimer's disease is widely believed to be the major contributor to the disease.The detection principle of this kit is fluorescence resonance energy transfer (FRET) method. A fluorescence donor group and a quenching group are divided at both ends of the substrate. The fluorescence energy is transferred from the fluorescence donor to the quenching group, resulting in the attenuation of the fluorescence intensity of the fluorescence donor molecule itself. When the substrate is cleaved by BACE 1, the quenching group of the substrate and the fluorescent donor are separated and the fluorescent donor is no longer quenched, the fluorescence of the fluorescent donor can be detected, so that the enzymatic activity of BACE 1 can be calculated. The fluorescence produced by this kit had a maximum excitation wavelength of 325 nm and a maximum absorption wavelength of 393 nm.
Product Categories/Family for BACE1 assay kit
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of BACE1 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for BACE1. It may not necessarily be applicable to this product.
Protein Family
Beta-secretase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.