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Canine TNF-alpha ELISA Kit
Canine TNF-alpha ELISA Kit
RayBio® Canine TNF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Canine (dog) |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 700 pg/ml |
| Specificity | This ELISA antibody pair detects canine TNF-alpha. Other species not determined yet. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P51742 |
| Gene Id | 403922 |
| Gene Symbols | TNF, TNFA, TNFSF2 |
| Protein Name / Synonyms | Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Canine TNF-alpha qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Canine TNF-alpha ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified TNF alpha (TNFA), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Canine diffuse large B-cell lymphoma downregulates the activity of CD8 + T-cells through tumor-derived extracellular vesicles
- Species:
- Dog
- Sample Type:
- extracellular vesicles (EVs)
- Year:
- 2023
- Journal Name:
- Cancer Cell International
Inflammaging in domestic dogs: basal level concentrations of IL-6, IL-1β, and TNF-α in serum of healthy dogs of different body sizes and ages
- Species:
- Dog
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Biogerontology
Novel Insights into Systemic Hyaluronic Acid Therapy in Dogs with Osteoarthritis from an Exploratory Postmarketing Study: Clinical Improvements Linked to Biomarker Changes
- Species:
- Canine
- Sample Type:
- Plasma (dogs with osteoarthritis)
- Year:
- 2025
- Journal Name:
- Animals
- PubMed ID:
- 41227470
A new combination of a prebiotic and postbiotic mitigates immunosenescence in vaccinated healthy senior dogs
- Species:
- Canine
- Sample Type:
- Serum (vaccinated senior dogs, D0/D28/D105)
- Year:
- 2024
- Journal Name:
- Frontiers in Veterinary Science
- PubMed ID:
- 39634761
Establishing a standardized biorepository across 3 clinical and translational science center-affiliated veterinary institutions is feasible and productive
- Species:
- Canine
- Sample Type:
- Urine (archived biorepository specimens)
- Year:
- 2026
- Journal Name:
- American Journal of Veterinary Research
- PubMed ID:
- 42114558
Clinical and inflammatory response to antiviral treatments in dogs with parvoviral enteritis
- Species:
- Canine
- Sample Type:
- Serum (dogs with parvoviral enteritis)
- Year:
- 2024
- Journal Name:
- Journal of Veterinary Science
- PubMed ID:
- 38311324
