Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.
 
> Cattle CCK(Cholecystokinin) ELISA Kit

Cattle CCK(Cholecystokinin) ELISA Kit

Afdrukken Afdrukken
Log in alstublieft

Referentie NB-66-25185-96T

Formaat : 96T

Merk : Neo Biotech


Products

Cattle CCK(Cholecystokinin) ELISA Kit
One-click to copy product information
ELK7599
Size： ：
48 T
96 T
96 T*5
Manual MSDS Send email to us

Overview

Product name: Cattle CCK(Cholecystokinin) ELISA Kit
Reactivity: Cattle
Alternative Names: CCK-PZ
Assay Type: Competitive Inhibition
Sensitivity: 4.96 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Tumor immunity;Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Uniprot ID: P41520
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle CCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.199
500.00 0.397
250.00 0.583
125.00 0.833
62.50 1.152
31.25 1.565
15.63 1.789
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CCK and the recovery rates were calculated by comparing the measured value to the expected amount of CCK in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 90%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 85-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 85-98% 89-101% 85-97%
EDTA plasma(n=5) 80-101% 87-96% 96-105% 95-103%
Heparin plasma(n=5) 87-96% 86-92% 95-103% 92-104%