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> Cav1.3 Antibody

Cav1.3 Antibody

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Referentie ASM10180-A680-100

Formaat : Onrequest

Merk : Abcepta

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  • IHC - Cav1.3 Antibody ASM10180
    Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium channel Monoclonal Antibody, Clone S38-8 (ASM10180). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV1.3 Calcium channel Monoclonal Antibody (ASM10180) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
  • WB - Cav1.3 Antibody ASM10180
    Western Blot analysis of Rat showing detection of ~250 kDa Cav1.3 protein using Mouse Anti-Cav1.3 Monoclonal Antibody, Clone S38-8 (ASM10180). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Tissue cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Cav1.3 Monoclonal Antibody (ASM10180) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~250 kDa.
  • IHC - Cav1.3 Antibody ASM10180
    Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S38-8 (ASM10180). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (ASM10180) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, AM
Primary Accession P27732
Other Accession NP_058994.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat Cav1.3 Monoclonal IgG1
Target/Specificity Detects ~250kDa. No cross-reactivity against Cav1.2.
Other Names alpha-1 polypeptide Antibody, CAC1D_HUMAN Antibody, CACH3 Antibody, CACN4 Antibody, CACNA 1D Antibody, Cacna1d Antibody, CACNL1A2 Antibody, Calcium channel Antibody, Calcium channel L type alpha 1 polypeptide isoform 2 Antibody, Calcium channel neuroendocrine/brain type alpha 1 subunit Antibody, Calcium channel voltage dependent L type alpha 1D subunit Antibody, CCHL1A2 Antibody, isoform 2 Antibody, L type Antibody, Voltage dependent L type calcium channel subunit alpha 1D Antibody, Voltage gated calcium channel alpha 1 subunit Antibody, Voltage gated calcium channel alpha subunit Cav1.3 Antibody, Voltage gated calcium channel subunit alpha Cav1.3 Antibody, Voltage-dependent L-type calcium channel subunit alpha-1D Antibody, Voltage-gated calcium channel subunit alpha Cav1.3 Antibody
Clone Names S38-8
Immunogen Fusion protein amino acids 2025-2161 of rat Cav1.3
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-302 was sufficient for detection of Cav1.3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane | Cell Membrane