CD206 antibody
Referentie orb308745-100ug
Formaat : 100ug
Merk : Biorbyt
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
CD206 antibody
Catalog Number: orb308745
| Catalog Number | orb308745 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD206 |
| Clonality | Monoclonal |
| Clone Number | 15-2 |
| Tested applications | FC, ICC, IHC-Fr, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Purified human mannose receptor |
| Concentration | 1 mg/ml |
| Dilution range | Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD206 |
| Entrez | 4360 |
| UniProt ID | P22897 |
| Storage | Store at 2-8°C. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti MMR antibody |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 μg/ml), GAM PE.
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (concentration in sample 9 μg/ml), GAM PE.
Western blotting analysis of human CD206 using mouse monoclonal antibody 15-2 on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD206 was detected around 170 kDa.