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> CD80 (B7-1) (FITC)

CD80 (B7-1) (FITC)

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Referentie 214743-FITC-100ul

Formaat : 100ul

Merk : US Biological


214743-FITC CD80 (B7-1) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Swiss Prot
O46405
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD80 cell surface antigen is expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells in the primary immune response.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: IL-A159|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD80. Species Crossreactivity: sheep ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD80. Species Crossreactivity: sheep
References
1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells. Scand J Immunol. 61: 242-50 2. Rhodes, S.G. et al. (2003) 1,25-Dihydroxyvitamin D3 and development of tuberculosis in cattle. Clin. Diag. Lab. Immunol. 10: 1129-35. 3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells. J. Gen. Virol. 84: 1771-1780. 4. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP– subset in lymph dendritic cells from the upper aero-digestive tract. J. Leukoc. Biol. 76:553-561. 5. Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node. J Leukoc Biol. 79: 268-76. 6. Hart, J. et al. (2011) Theileria annulata-transformed cell lines are efficient antigen-presenting cells for in vitro analysis of CD8 T cell responses to bovine herpesvirus-1. Vet Res. 42: 119. 7. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro. Vet Res. 44: 59.