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CENP-A Antibody

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Referentie ASM10149-A655-100

Formaat : Onrequest

Merk : Abcepta

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  • ICC/IF - CENP-A Antibody ASM10149
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 (ASM10149). Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody (ASM10149) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
  • WB - CENP-A Antibody ASM10149
    Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 (ASM10149). Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody (ASM10149) at 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P49450
Other Accession NP_001035891.1
Host Mouse
Isotype IgG1
Reactivity Human
Clonality Monoclonal
Description Mouse Anti-Human CENP-A Monoclonal IgG1
Target/Specificity Detects ~18kDa. Recognizes Human CENP-A.
Other Names CENP A Antibody, cenpa Antibody, Centromere auto antigen A Antibody, Histone H3 like centromeric protein A Antibody
Clone Names 5A7-2E11
Immunogen Synthetic peptide corresponding to a portion of human CENP-A
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Nucleus | Chromosome | Centromere | Kinetochore