CGA ELISA Kit (Fish)
Referentie OKEH03759
Formaat : 96Wells
Merk : Aviva Systems Biology
CGA ELISA Kit (Fish) (OKEH03759)
| Datasheets/Manuals | Printable datasheet for CGA ELISA Kit (Fish) (OKEH03759) |
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| Predicted Species Reactivity | Fish|Oryzias latipes | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology CGA ELISA Kit (Fish) (OKEH03759) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CGA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CGA captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50mIU/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.32mIU/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Fish Glycoprotein hormones alpha chain | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | CGA |
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| Gene Full Name | glycoprotein hormones, alpha polypeptide |
| Alias Symbols | zgc:77358 |
| NCBI Gene Id | 402928 |
| Protein Name | Glycoprotein hormones alpha chain |
| Description of Target | Glycoprotein hormones alpha chain |
| Uniprot ID | Q6QX43 |
| Protein Accession # | NP_991250.1 |
| Nucleotide Accession # | NM_205687.2 |
| Protein Size (# AA) | 117 |
| Molecular Weight | 13 kDa |
