Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Cholesterol oxidase microorganism in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Reactivity
Bacteria
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Cholesterol oxidase from Streptomyces
Specificity
This product detects Cholesterol oxidase (microorganism). Cross reactivity against Cholesterol oxidase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Cholesterol oxidase microorganism.
Restore with 0.1 ml of deionized water (or equivalent).
Concentration
lot specific
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation
Biotin
Storage
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability
Shelf life: one year from despatch.
Shipping
Ambient
Background
Cholesterol oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
