Choline is a water-soluble nutrient. Acetylcholine (ACh) acts as a neurotransmitter in the autonomic nervous system (ANS). Choline and ACh play important roles in many biological processes.

The Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit provides a highly sensitive, fast and convenient way for detection of choline and acetylcholine in various biological samples such as blood, cells, fermentation media and culture media based on colorimetric and fluorometric method. In the assay, free choline is oxidized to betaine via the intermediate betaine aldehyde. The reaction generates products which react with Choline Probe to yield fluorescence (Ex/Em 535/587 nm) and color (λ = 570 nm). Acetylcholine is converted to choline by acetylcholinesterase. The assay can detect choline and acetylcholine (total choline-free choline). The kit can detect 10 pmol-5 nmol of choline or acetylcholine without pretreatment or purification of samples.