Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Clotrimazole [23593-75-1]

Afdrukken

Referentie NB-64-00682-500mg

Formaat : 500mg

Merk : Neo Biotech

Meer informatie aanvragen

Datasheet (EN)

Remove All

Clotrimazole

(Synonyms: FB 5097, BAY b 5097) Copy Product Info

Synonyms: FB 5097, BAY b 5097

Catalog No. T0506 Copy Product Info

Purity: 99.89%

Clotrimazole (FB 5097), an imidazole derivative with a broad spectrum of antimycotic activity, inhibits biosynthesis of the sterol ergostol.

Cas No. 23593-75-1

For research use only—not for human use. No sales to individuals. Use as intended only.

Select Batch

Purity:99.89%

Appearance:Solid

Color:White

Product Information

Bioactivity

Description	Clotrimazole (FB 5097), an imidazole derivative with a broad spectrum of antimycotic activity, inhibits biosynthesis of the sterol ergostol.
In vitro	Intradermal injection of Clotrimazole in mice stimulates capsaicin-sensitive and mustard oil-sensitive trigeminal neurons, inducing nocifensive behavior and thermal hyperalgesia. In TRPV1 knockout mice, Clotrimazole-induced pain behavior is inhibited by the TRPV1 antagonist BCTC [1,2,3,4-tetrahydro-6-trichloromethyl-pyrimidine-2-one].
In vivo	In the CRI-G1 whole-cell of a rat insulinoma cell line, Clotrimazole antagonizes ADP-ribose-activated and single-channel currents. Clotrimazole effectively and rapidly inhibits parasite growth across five different strains of the malignant malaria protozoan. In HEK-293 cells expressing recombinant human TRPM2, Clotrimazole inhibits ADP-ribose-activated currents. At 2 mM concentration, Clotrimazole completely suppresses parasite replication, resulting in the destruction of host cells. Furthermore, Clotrimazole at concentrations ranging from 3 mM to 30 mM entirely inhibits TRPM2-mediated currents.
Synonyms	FB 5097, BAY b 5097

Chemical Properties

Molecular Weight	344.84
Formula	C₂₂H₁₇ClN₂
Cas No.	23593-75-1
Smiles	Clc1ccccc1C(c1ccccc1)(c1ccccc1)n1ccnc1
Relative Density.	1.1095 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 36.43 mg/mL (105.64 mM), Sonication is recommended.

Ethanol: 64 mg/mL (185.59 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.9 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.8999 mL	14.4995 mL	28.9990 mL	144.9948 mL
5 mM	0.5800 mL	2.8999 mL	5.7998 mL	28.9990 mL
10 mM	0.2900 mL	1.4499 mL	2.8999 mL	14.4995 mL
20 mM	0.1450 mL	0.7250 mL	1.4499 mL	7.2497 mL
50 mM	0.0580 mL	0.2900 mL	0.5800 mL	2.8999 mL
100 mM	0.0290 mL	0.1450 mL	0.2900 mL	1.4499 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

InhibitorinhibitFungalFB-