COLGALT1 ELISA Kit (Human)
Referentie OKEH04710
Formaat : 96Wells
Merk : Aviva Systems Biology
COLGALT1 ELISA Kit (Human) (OKEH04710)
| Datasheets/Manuals | Printable datasheet for COLGALT1 ELISA Kit (Human) (OKEH04710) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology COLGALT1 ELISA Kit (Human) (OKEH04710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COLGALT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COLGALT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COLGALT1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
| Sensitivity | 0.05 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Procollagen galactosyltransferase 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | COLGALT1 |
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| Gene Full Name | collagen beta(1-O)galactosyltransferase 1 |
| Alias Symbols | BSVD3, ColGalT 1, Collagen beta(1-O)galactosyltransferase 1, GLT25D1, glycosyltransferase 25 domain containing 1, glycosyltransferase 25 family member 1, hydroxylysine galactosyltransferase 1, procollagen galactosyltransferase 1. |
| NCBI Gene Id | 79709 |
| Protein Name | Procollagen galactosyltransferase 1 |
| Description of Target | Beta-galactosyltransferase that transfers beta-galactose to hydroxylysine residues of type I collagen (PubMed:19075007, PubMed:22216269, PubMed:27402836). By acting on collagen glycosylation, facilitates the formation of collagen triple helix (PubMed:27402836). |
| Uniprot ID | Q8NBJ5 |
| Protein Accession # | NP_078932.2 |
| Nucleotide Accession # | NM_024656.3 |
| Protein Size (# AA) | 622 |
| Molecular Weight | 72 kDa |