Formaat : 1PlateKit
COVID-19 Spike Variant-ACE2 Binding Assay Kit
The RayBio® COVID-19 Spike Variant-ACE2 binding assay kits are a rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein variants and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.
Positive and negative controls for the Spike-ACE2 binding assays are available as an accessory item. Click here.
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, SARS-CoV-2 |
| Compatible Sample Types | Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides |
| Accession Number | QHD43416 |
| Gene Symbols | ACE2, S, RBD, SRBD, S-RBD |
| Protein Name / Synonyms | Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2, Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD) |
| Format | Spike on plate + ACE2 in solution |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Protein Therapeutics, Receptor-Ligand Binding, Infectious Disease, COVID-19 |
| Shipping Type | Blue ice |
| Storage | 4°C |
| Note | The binding assay may be available in two different formats based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study. |
| Spike Variant | D614G: present in Alpha (B.1.1.7) variant, E484K: present in multiple variants, N501Y: present in multiple variants, K417T, K417N, L452R, Multiple mutations: Omicron (B.1.1.529) variant, HV69-70del |
The kit may be stored at 4°C up to 1 month from the date of shipment. The life of the kit can be extended up to 6 months from the date of shipment by storing the kit reagents (Item F, C, and D) at -20°C. All other components should be stored at 4°C.
Multiple mutations of the wild type SARS-CoV-2 Spike protein (Accession no. QHD43416) have emerged since the COVID-19 pandemic began. RayBiotech offers inhibitor screening ELISA kits featuring the Spike RBD protein with mutations that appear in major SARS-CoV-2 variants of concern. The binding of the RBD to ACE2 enables viral entry.
SARS-CoV-2 Variant Classifications and Definitions
RayBiotech spike RBD mutant binding assays include:
N501Y mutant: present in Alpha, Beta, and Gamma variants (B.1.1.7, B.1.351, P.1)
Spike RBD protein with one amino acid mutation: N501Y.
E484K mutant: present in Beta and Gamma variants (B.1.351, P.1)
Spike RBD protein with one amino acid mutation: E484K.
Beta variant mutations (B.1.351)
Spike RBD protein with three amino acid mutations: K417N, E484K, N501Y.
Gamma variant mutations (P.1)
Spike RBD protein with three amino acid mutations: K417T, E484K, N501Y.
D614G mutant: present in Alpha, Beta, and Gamma variants (B.1.1.7, B.1.351, P.1)
Spike RBD protein with one amino acid mutation: D614G.
Alpha variant mutations (B.1.1.7)
Spike S1 protein mutations include a deletion in NTD at HV69-70 and two amino acid mutations in RBD: N501Y, D614G.
Delta variant mutations (B.1.617.2)
Spike RBD protein with 2 amino acid mutations: L452R, T478K.
Omicron variant mutations (B.1.1.529)
Spike RBD protein with 15 amino acid mutations: G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H
Check out our binding assay using the wild-type Spike variant here.
