DNA Polymerase theta Antibody
Referentie MBS9612322-0,1mL
Formaat : 0.1mL
Merk : MyBiosource
MBS9612322 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612322) and mouse anti-beta tubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).
Western blot analysis of extracts from Mouse spleen, using DNA Polymerase theta Ab. The lane on the left was treated with blocking peptide.
MBS9612322 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab
DNA Polymerase theta (POLQ) Polyclonal Antibody – Human, Mouse, Rat Reactivity
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9612322MBS9612322 is an antibody targeting POLQ. Also known as DNA Polymerase theta Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
DNA Polymerase theta (POLQ), Polyclonal Antibody
Full Product Name
DNA Polymerase theta Antibody
Product Gene Name
anti-POLQ antibody
Product Synonym Gene Name
POLH
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific)
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
DNA Polymerase theta peptide (MBS9622266) is used for blocking the activity of DNA Polymerase theta antibody (MBS9612322)
Molecular Weight
289kDa, 150kD
OMIM
604419
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human DNA Polymerase theta, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-POLQ antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes for anti-POLQ antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
The optimal dilutions should be determined by the end user
IMPORTANT:
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
The optimal dilutions should be determined by the end user
IMPORTANT:
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunofluorescence (IF) of anti-POLQ antibody
MBS9612322 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612322) and mouse anti-beta tubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).
Immunohistochemistry (IHC) of anti-POLQ antibody
MBS9612322 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab
Other Notes
Small volumes of anti-POLQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for POLQ. It may not necessarily be applicable to this product.
NCBI Official Full Name
DNA polymerase theta
NCBI Official Synonym Full Names
DNA polymerase theta
NCBI Official Symbol
POLQ
NCBI Official Synonym Symbols
PRO0327
UniProt Protein Name
DNA polymerase theta
UniProt Synonym Protein Names
DNA polymerase eta
Protein Family
DNA polymerase
UniProt Gene Name
POLQ
UniProt Synonym Gene Names
POLH
UniProt Entry Name
DPOLQ_HUMAN
NCBI Protein Information
DNA polymerase theta
UniProt Comments for POLQ
POLQ: Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links. Belongs to the DNA polymerase type-A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Transferase; EC 2.7.7.7
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: nucleoplasm
Molecular Function: DNA-directed DNA polymerase activity; damaged DNA binding; ATP binding; single-stranded DNA-dependent ATPase activity
Biological Process: DNA repair; DNA-dependent DNA replication
Show more Protein type: DNA repair, damage; Transferase; EC 2.7.7.7
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: nucleoplasm
Molecular Function: DNA-directed DNA polymerase activity; damaged DNA binding; ATP binding; single-stranded DNA-dependent ATPase activity
Biological Process: DNA repair; DNA-dependent DNA replication
Research Articles on POLQ
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Diseases associated with anti-POLQ antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >9 publications with POLQ and Neoplasms |
| Breast Neoplasms Antibodies | >6 publications with POLQ and Breast Neoplasms |
| Drug Toxicity Antibodies | >2 publications with POLQ and Drug Toxicity |
| Lymphoma Antibodies | >1 publications with POLQ and Lymphoma |
| Neoplasms, Experimental Antibodies | >1 publications with POLQ and Neoplasms, Experimental |
| Pancreatic Neoplasms Antibodies | >1 publications with POLQ and Pancreatic Neoplasms |
| Anemia Antibodies | >1 publications with POLQ and Anemia |
Organs/Tissues associated with anti-POLQ antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >10 publications with POLQ and Blood |
| Testis Antibodies | >3 publications with POLQ and Testis |
| Lymph Antibodies | >2 publications with POLQ and Lymph |
| Thymus Antibodies | >2 publications with POLQ and Thymus |
| Lymph Node Antibodies | >2 publications with POLQ and Lymph Node |
| Bone Antibodies | >1 publications with POLQ and Bone |
| Brain Antibodies | >1 publications with POLQ and Brain |
| Lung Antibodies | >1 publications with POLQ and Lung |
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