Dopamine ELISA Kit
Referentie OKCD02264
Formaat : 96Wells
Merk : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Dopamine ELISA Kit (OKCD02264) |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dopamine (DA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dopamine (DA) and unlabeled Dopamine (DA) (Standards or samples) with the pre-coated antibody specific to Dopamine (DA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dopamine (DA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dopamine (DA) in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 12.35-1,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine (DA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine (DA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 51-61-6 PubChem: 681 | ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 4.71 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | DA |
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Alias Symbols | Dopamine; 4-(2-Aminoethyl)benzene-1,2-diol; Hydroxytyramin; Oxytyramine; Dopamin |
Protein Name | Dopamine |
Description of Target | One of the catecholamine NEUROTRANSMITTERS in the brain. Dopamine is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action. |