EGFR Antibody
Referentie OAEE01031
Formaat : 100tests(T100)
Merk : Aviva Systems Biology
| Datasheets/Manuals | Printable datasheet for EGFR Antibody |
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| Predicted Species Reactivity | Horse|Human|Mouse|Rat |
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| Product Format | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Clonality | Monoclonal |
| Clone | EGFR1 |
| Isotype | IgG2b |
| Host | Mouse |
| Conjugation | R-PE |
| Application | FC |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Human epidermoid carcinoma line A431 |
| Purification | Purified by size-exclusion chromatography |
| Concentration | 10 ul / test |
| Specificity | The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1); epitope within amino acids 6-273. |
| Formulation | PE |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
| Reference | Fortunel NO, Hatzfeld JA, Rosemary PA, Ferraris C, Monier MN, Haydont V, Longuet J, Brethon B, Lim B, Castiel I, Schmidt R, Hatzfeld A: Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations. J Cell Sci. 2003 Oct 1;116(Pt 19):4043-52. Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936. Akiyama M, Smith LT, Shimizu H: Changing patterns of localization of putative stem cells in developing human hairfollicles. J Invest Dermatol. 2000 Feb;114(2):321-7. Aigner A, Juhl H, Malerczyk C, Tkybusch A, Benz CC, Czubayko F: Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation. Oncogene. 2001 Apr 19;20(17):2101-11. |
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| Gene Symbol | EGFR |
| Gene Full Name | epidermal growth factor receptor |
| Alias Symbols | avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor tyrosine kinase domain, erb-b2 receptor tyrosine kinase 1, ERBB, ERBB1, ERRP, HER1, mENA, NISBD2, PIG61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1. |
| NCBI Gene Id | 1956 |
| Protein Name | Epidermal growth factor receptor |
| Description of Target | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance (By similarity). |
| Uniprot ID | P00533 |
| Protein Accession # | NP_005219.2; NP_958439.1; NP_958440.1; NP_958441.1 |
| Nucleotide Accession # | NM_005228.3; NM_201282.1; NM_201283.1; NM_201284.1 |
