ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, Guinea Pig complement (at a 1/60 dilution) and 2% RBC, not less than 1/500 when tested with Bovine Erythrocytes.
Reactivity
Bovine
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Washed whole Erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
Bovine Erythrocytes. To sensitize cells in Haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes. Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified Hyperimmune IgG fraction
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation
Unconjugated
Storage
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
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