Formaat : 2g
Synonyms: β-Estradiol, E2, 17β-Oestradiol, 17β-Estradiol
| Description | Estradiol (E2) is a naturally occurring steroidal sex hormone that is essential for female fertility and maintenance of secondary sexual characteristics. Estradiol upregulates IL-6 expression through estrogen receptor beta (ERβ). |
| Targets & IC50 | ERα:22-28 nM, ERα (human):22-28 nM, ERα (mouse):22-28 nM |
| In vitro | METHODS: Human lens epithelial cells HLEC were treated with Estradiol (0.001-10 μM) for 24 h. Cell viability was measured using the MTT. RESULTS: The number of viable cells was significantly reduced in the presence of 10 μM Estradiol. [1] METHODS: Human breast cancer cells MB231 were treated with Estradiol (100 nM) for 24 h. Cell motility was detected using scratch/wound healing assay and transwell assay. RESULTS: Estradiol treatment reduced the migration and invasion of MB231 cells. [2] |
| In vivo | METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) and progesterone (5-20 mg/kg) were administered intraperitoneally as a single injection to ovariectomized C57BL/6 female mice. RESULTS: Mice receiving Estradiol alone or Estradiol plus 10-20 mg/kg progesterone showed significantly enhanced memory for new objects. [3] METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) was administered by gavage to ovariectomized C57BL/6 female mice once daily for two weeks. RESULTS: Long-term activation of ERβ reduced hot flash-like symptoms and enhanced spatial and object recognition memory in ovariectomized mice. [4] |
| Synonyms | β-Estradiol, E2, 17β-Oestradiol, 17β-Estradiol |
| Cell Research | Estradiol is dissolved in 0.01% ethanol. Briefly, B9 cells (5×103/well of a 96-well plate) are cultured with a series of dilutions of the supernatants in a final volume of 100 mL of RPMI 1640, supplemented with 5×10-5mol/Lof 2-mercaptoethanol, 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin in flat-bottom microtiter plates. After 42 h, 0.5 μCi of [3H]thymidine is added. 6 h later, the cells are harvested and the radioactivity incorporated is determined. IL-6 is quantitated from a standard curve set up with known amounts of recombinant human or mouse IL-6. The anti-IL-6 monoclonal antibody completely inhibited the ability of the B9 cells to proliferate in response to recombinant mouse IL-6. In addition, we determined that B9 cells do not proliferate in response to IL-I or TNF, and that antibodies to these two cytokines did not affect the cell proliferation in response to IL-6. Further, it is established that neither Estradiol (17β-estradiol), nor the 0.01% ethanol used as vehicle in these experiments, has any effect on the bioassay. |
| Molecular Weight | 272.38 |
| Formula | C18H24O2 |
| Cas No. | 50-28-2 |
| Smiles | C[C@@]12[C@]([C@]3([C@@](C=4C(CC3)=CC(O)=CC4)(CC1)01)01)(CC[C@@H]2O)01 |
| Relative Density. | 1.17g/cm3 |
| Storage | Keep away from direct sunlight,Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: <1 mg/mL (insoluble)  Ethanol: 20.4 mg/mL (74.9 mM), Sonication is recommended. DMSO: 250 mg/mL (917.84 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2.73 mg/mL (10.02 mM), Suspension. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
Ethanol/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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