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> Frizzled 5 Protein, Human, Recombinant (His)

Frizzled 5 Protein, Human, Recombinant (His)

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Frizzled 5 Protein, Human, Recombinant (His)

(Synonyms: HFZ5, frizzled class receptor 5, C2orf31) Copy Product Info

Synonyms: HFZ5, frizzled class receptor 5, C2orf31

Catalog No. TMPY-01469 Copy Product Info
Frizzled 5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.6 kDa and the accession number is Q13467.
Other Forms of Frizzled 5 Protein, Human, Recombinant (His):
Frizzled 5 Protein, Mouse, Recombinant (hFc)
Frizzled 5 Protein, Human, Recombinant (hFc)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 96.2%; SEC-HPLC: 96.4%
Appearance:Lyophilized powder
Color:White
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Product Information

Bioactivity
Bioactivity
Immobilized Recombinant Human Frizzled-5 / FZD5 Protein (His Tag) at 2μg/mL (100μL/well) can bind pan-FZD antibody, Human IgG2 , the EC50 is 12-36 ng/mL.
Description
Frizzled 5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.6 kDa and the accession number is Q13467.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberQ13467
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Pro 167) of human frizzled 5 (NP_003459.2) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 27
Protein Purity
≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Frizzled 5 Protein, Human, Recombinant (His),Frizzled 5 Protein, Human, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsHFZ5, frizzled class receptor 5, C2orf31
Research Background
Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 1 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt1B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.
Chemical Properties
Molecular Weight17.6 kDa (predicted); 25-30 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

HFZ-5HFZ 5
Related Tags: Frizzled 5 Protein, Human, Recombinant (His) chemical structure | Frizzled 5 Protein, Human, Recombinant (His) molecular weight