Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

GANT 61 [500579-04-4]

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Referentie NB-64-19479-1mL

Formaat : 1mLx10mM(inDMSO)

Merk : Neo Biotech

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GANT 61

(Synonyms: NSC 136476, GANT61) Copy Product Info

Synonyms: NSC 136476, GANT61

Catalog No. T3070 Copy Product Info

Purity: 99.62%

GANT 61 (GANT61) is an inhibitor for Gli1 and Gli2.

Cas No. 500579-04-4

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.62%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	GANT 61 (GANT61) is an inhibitor for Gli1 and Gli2.
Targets & IC50	GLI1:5 μM
In vitro	Nude mice bearing SK-N-AS neuroblastoma xenografts administered orally with 50 mg/kg GANT61 exhibited significant tumor growth inhibition on day 12 of the experiment, achieving a 63% reduction in tumor volume compared to the control group. Additionally, in nude mice injected with GLI1-positive 22Rv1 prostate cancer cells, GANT61 induced tumor regression to the point where no discernible tumors were observed.
In vivo	GANT61 effectively inhibits tumor cell proliferation in vitro through a GLI-dependent mechanism. At a concentration of 30μM, GANT61 halts growth and induces apoptosis in acute myeloid leukemia cells. It exhibits potent cytotoxicity towards human colon cancer cell lines, eradicating colony formation. GANT61 specifically targets chronic lymphocytic leukemia cells over normal B lymphocytes, triggering apoptosis. In the early S phase of human colon cancer cell lines, GANT61 induces DNA replication inhibition leading to DNA damage signaling through the ATM-Chk2 axis and consequent cell death. Furthermore, GANT61 inhibits GLI1's DNA binding capability and suppresses the hedgehog signaling pathway with an IC50 of 5μM, displaying higher selectivity over other pathways (such as TNF signaling/NFκB activation, glucocorticoid receptor gene transcription, and the Ras-Raf-Mek-Mapk cascade).
Synonyms	NSC 136476, GANT61
Kinase Assay	Dual Luciferase Assay: HEK293 cells are transfected with GLI1 expression plasmid, together with the reporter plasmids 12×GliBSLuc and R-Luc on 10-cm plates (day 0). Twenty-four hours later, cells are seeded in white 96-well plates with clear bottom at a density of 15,000 cells per well. Cells are allowed to attach, and compounds are added at a final concentration of 10 μM in DMSO (0.5% final DMSO concentration) (day 1.5). Cells are grown for another 24 h, subsequently lysed, and then analyzed by using the Dual Luciferase kit.
Cell Research	BrdU Incorporation Assay. Subconfluent cells are grown in reduced FBS (2.5%) for 48 h in the presence of 5 μM test compound (or DMSO) on white 96-well plates with clear bottom. Subsequently, cells are labeled for 2 h with BrdU, fixed, and analyzed.(Only for Reference)

Chemical Properties

Molecular Weight	429.6
Formula	C₂₇H₃₅N₅
Cas No.	500579-04-4
Smiles	CN(C)c1ccccc1CN1CCCN(Cc2ccccc2N(C)C)C1c1ccncc1
Relative Density.	1.134 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 25 mg/mL (58.19 mM), Sonication is recommended.

Ethanol: 43 mg/mL (100.09 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.33 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.3277 mL	11.6387 mL	23.2775 mL	116.3873 mL
5 mM	0.4655 mL	2.3277 mL	4.6555 mL	23.2775 mL
10 mM	0.2328 mL	1.1639 mL	2.3277 mL	11.6387 mL
20 mM	0.1164 mL	0.5819 mL	1.1639 mL	5.8194 mL
50 mM	0.0466 mL	0.2328 mL	0.4655 mL	2.3277 mL

Ethanol

	1mg	5mg	10mg	50mg
100 mM	0.0233 mL	0.1164 mL	0.2328 mL	1.1639 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

SmoothenedNSC-136476NSC136476InhibitorinhibitHedgehogGLI1GliGANT-61GANT 61Autophagy

Related Tags: GANT 61 chemical structure | GANT 61 in vivo | GANT 61 in vitro | GANT 61 formula | GANT 61 molecular weight