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> GluA1/GluR1 Glutamate Receptor Antibody

GluA1/GluR1 Glutamate Receptor Antibody

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Referentie ASM10274-A680-100

Formaat : Onrequest

Merk : Abcepta

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Telefoonnummer : +1 850 650 7790

 
  • ICC/IF - GluA1/GluR1 Glutamate Receptor Antibody ASM10274
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody, Clone S355-1 (ASM10274). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody (ASM10274) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluA1/GluR1 Glutamate Receptor Antibody (D) Composite.
  • WB - GluA1/GluR1 Glutamate Receptor Antibody ASM10274
    Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluA1-GluR1 protein using Mouse Anti-GluA1-GluR1 Monoclonal Antibody, Clone S355-1 (ASM10274). Load: 10 µg. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluA1-GluR1 Monoclonal Antibody (ASM10274) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession P19490
Other Accession NP_113796
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GluA1/GluR1 Glutamate Receptor Monoclonal IgG1
Target/Specificity Detects ~100kDa. Does not cross-react with GluR2.
Other Names AMPA 1 Antibody, AMPA selective glutamate receptor 1 Antibody, Glutamate receptor 1 Antibody, GluA1 Antibody, GLUH 1 Antibody, GluR K1 Antibody, GluRK1 Antibody, Glr-1 Antibody, Glur-1 Antibody, HIPA Antibody, Glur1 Antibody, GluR-A Antibody, AI853806 Antibody, 2900051M01Rik Antibody, GluRA Antibody, Glr1 Antibody, MGC13325 Antibody, GLUH1 Antibody, GluA1 Antibody, GLUR1 Antibody, HBGR1 Antibody, GLURA Antibody, gluR- Antibody, GluA1 Antibody, Glutamate receptor ionotropic AMPA 1 Antibody, Glutamate receptor ionotropic Antibody, Gria 1 Antibody, HBGR1 Antibody, OTTHUMP00000160643 Antibody, OTTHUMP00000165781 Antibody, OTTHUMP00000224241 Antibody, OTTHUMP00000224242 Antibody, OTTHUMP00000224243 Antibody
Clone Names S355-1
Immunogen Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-440 was sufficient for detection of GluA1/GluR1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Endoplasmic Reticulum | Cell Junction