Griffonia simplicifolia GS II

The seeds of Griffonia simlicifolia conain four distinct lectins, 30,000D each. Two of these lectins, referred to as GS-I and GS-II have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer but it is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only a terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding.

Source:
Bandeiraea

Inhibiting/Eluting Sugar:
Chitin Hydrolysate or 200mM N-acetylglucosamine

Activity:
5-10ug/ml will agglutinate Tk polyagglutinaable cells.

Inhibitory Carbohydrate:
N-acetylglucosamine

Carbohydrate Specificity:
Terminal a- or b- N- Acetylglucosamine. The specific linkage of N-Acetylglucosamine to the subterminal carbohydrate plays an important role in lectin binding.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.