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> GRP78 Antibody

GRP78 Antibody

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Referentie ASM10156-A680-100

Formaat : Onrequest

Merk : Abcepta

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Telefoonnummer : +1 850 650 7790

 
  • ICC/IF - GRP78 Antibody ASM10156
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11 (ASM10156). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (ASM10156) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen . Magnification: 60X.
  • WB - GRP78 Antibody ASM10156
    Western Blot analysis of Human, Mouse, Rat HEK-293, NIH3T3, and Rat Brain cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11 (ASM10156). Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (ASM10156) at 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, E
Primary Accession P06761
Other Accession NP_037215.1
Host Mouse
Isotype IgG1 Kappa
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa
Target/Specificity Detects ~78kDa.
Other Names 78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regu 0   <2 Antibody
Clone Names 3G12-1G11
Immunogen Full-length recombinant rat GRP78
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-211 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome