Origin:

Cervix carcinoma

Origin:

Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining.

Species:

Homo sapiens, human

Tissue:

HeLa derivative, human cervix carcinoma

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Modal no. 76

Patient:

Female, Black

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (85%) at 1:3 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO