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> His-Column High Density Nickel

His-Column High Density Nickel

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Referentie 6BCL-QHNi-C8

Formaat : 8x1ml

Merk : Agarose Bead Technologies (ABT)


High Density Nickel

Product info

Cat. No.
6BCL-QHNi-X
Bead Geometry & Size
Spherical, Standard: ~50 – 150 µm
Crosslinked
Yes
Agarose %
6 %
Antimicrobial Agent
20% Ethanol
Storage Temperature
2-8ºC
Product Name
High Density Nickel
Matrix
Stable in all commonly used reagents
Binding/Loading Capacity (μmol Me2+/ml gel)
20-40
6BCL-QHNi-25

Overview

Resume

Nickel chelate resins recognize two exposed histidine for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins.

HIGH Density NICKEL provides a higher amount of available groups and usually allows for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow).

ABT offers 25ml 100ml and 500ml For Bulk quantities click here.

Description

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins.
High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow).

REFERENCES

  • Lechner, Horst et al. “Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations.” Biotechnology Journal 13.3 (2018): n. pag. Biotechnology Journal. 
  • Marsden, A. P. et al. Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome. Journal of Molecular Biology 430, 5207–5216 (2018).
  • Sanchez-Rodriguez, Ana et al. “Recombinant Rabbit Beta Nerve Growth Factor Production and Its Biological Effects on Sperm and Ovulation in Rabbits.” PLoS ONE 14.7 (2019): n. pag. PLoS ONE. 

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