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> Histone H3, phosphorylated (Ser10) (Mitosis Marker) (APC)

Histone H3, phosphorylated (Ser10) (Mitosis Marker) (APC)

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Referentie H5110-14B-APC-100ul

Formaat : 100ul

Merk : US Biological


H5110-14B-APC Histone H3, phosphorylated (Ser10) (Mitosis Marker) (APC)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
Q16695
Isotype
IgG
Grade
Affinity Purified
Applications
IC IP WB
Crossreactivity
Dr Hu Th Xe
Accession #
NP_003484.1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-47H: IgG, H&L (HRP) Pab GtxRt
I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt
I1904-47T: IgG, H&L (HRP) Pab GtxRt
I1904-50T1: IgG, H&L (HRP) Pab GtxRt
I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt
I1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
I1904-46N: IgG, H&L (FITC) Pab Gt xRb

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Linear peptide coresponding to human Histone H3 at Ser10|Specificity: Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Linear peptide coresponding to human Histone H3 at Ser10
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
References
1. Park, K.-S. et al., (2006) Am J Respir Cell Mol Biol. 34:151–157. 2. Akeno N., et al. 2014. Oncogene. 2014 Jan 27. doi: 10.1038/onc.2013.589. [Epub ahead of print].|Other references:|1. Adams, R.R., et al., J. Cell Biol. 153: 865-880 (2001). 2. Canela, N., et al., J. Biol. Chem. 278: 1158-1164 (2003). 3. Seigel, P.M. et al., Proc Natl. Acad. Sci. USA 100: 8430-8435 (2003). 4. Wei, Y., et al., Cell 97: 99-109 (1999) 5. Miller, L.D., et al., Developmental Dynamics 231: 57-71 (2004). 6. Chadee, D.N., et al., J. Biol. Chem. 270: 20,098-20,105 (1995). 7. Ajiro, K., et al., J. Biol. Chem. 271: 13,197-13,201 (1996). 8. Mahadevan, L.C., et al., Cell 65: 775-783 (1991). 9. Hendzel, M.J., et al., Chromasoma 106: 348-360 (1997).