Histone H3K9me3 Polyclonal Antibody
Referentie MBS126185-0,02mL
Formaat : 0.02mL
Merk : MyBiosource
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.
Immunohistochemistry of paraffin-embedded rat lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TriMethyl-Histone H3-K9 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse lung using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (MBS126185) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
mmunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (MBS126185) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (MBS126185) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti- Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF7 cells using TriMethyl-Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti- Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl- Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti- Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (MBS126185) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input
H3K9me3 Antibody – Rabbit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126185SPECIAL PROMOTION: Get FREE Amazon gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin… More details
Product Overview
Online Datasheet in PDFProduct Name
H3K9me3, Antibody
Full Product Name
Histone H3K9me3 Polyclonal Antibody
Product Gene Name
anti-H3K9me3 antibody
Product Synonym Names
TriMethyl-Histone H3-K9: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 17KDa
Calculated MW: 15kDa
Calculated MW: 15kDa
OMIM
602820
Source
Human
Immunogen
Synthetic peptide
Species Reactivity/Cross Reactivity
Human, Mouse, Rat, Other (Wide Range Predicted)
Product Categories/Family for anti-H3K9me3 antibody
Methylation
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H3K9me3 antibody
Dot Blot (DB), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Chromatin Immunoprecipitation (CHIP), Immunocytochemistry (ICC)
Application Notes for anti-H3K9me3 antibody
WB: 1:100 - 1:500
DB: 1:500 - 1:2000
IHC-P: 1:50 - 1:200
IF/ICC: 1:50 - 1:200
ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.
ChIP: 2.5ug antibody for 5ug-10ug of Chromatin
DB: 1:500 - 1:2000
IHC-P: 1:50 - 1:200
IF/ICC: 1:50 - 1:200
ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.
ChIP: 2.5ug antibody for 5ug-10ug of Chromatin
Western Blot (WB) of anti-H3K9me3 antibody
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Chromatin Immunoprecipitation (CHIP) of anti-H3K9me3 antibody
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (MBS126185) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunofluorescence (IF) of anti-H3K9me3 antibody
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti- Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence (IF) of anti-H3K9me3 antibody
Immunofluorescence analysis of MCF7 cells using TriMethyl-Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti- Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence (IF) of anti-H3K9me3 antibody
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl- Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti- Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence (IF) of anti-H3K9me3 antibody
Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K9 Rabbit pAb (MBS126185) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation (CHIP) of anti-H3K9me3 antibody
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (MBS126185) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input
Other Notes
Small volumes of anti-H3K9me3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for H3K9me3 purchase
MBS126185 is an antibody targeting H3K9me3. Also known as Histone H3K9me3 Polyclonal Antibody. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for H3K9me3. It may not necessarily be applicable to this product.
NCBI Official Full Name
Histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
UniProt Protein Name
Histone H3.1t
UniProt Synonym Protein Names
H3/g
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
NCBI Summary for H3K9me3
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for H3K9me3
HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
Show more Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
Research Articles on H3K9me3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-H3K9me3 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Alcoholism Pathway antibodies | Alcoholism Pathway Diagram |
| Alcoholism Pathway antibodies | Alcoholism Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle, Mitotic Pathway antibodies | Cell Cycle, Mitotic Pathway Diagram |
| Cellular Senescence Pathway antibodies | Cellular Senescence Pathway Diagram |
| Cellular Responses To Stress Pathway antibodies | Cellular Responses To Stress Pathway Diagram |
| Chromosome Maintenance Pathway antibodies | Chromosome Maintenance Pathway Diagram |
| Condensation Of Prophase Chromosomes Pathway antibodies | Condensation Of Prophase Chromosomes Pathway Diagram |
| DNA Damage/Telomere Stress Induced Senescence Pathway antibodies | DNA Damage/Telomere Stress Induced Senescence Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
Diseases associated with anti-H3K9me3 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Fibrosis Antibodies | >1 publications with H3K9me3 and Fibrosis |
| Cell Transformation, Neoplastic Antibodies | >1 publications with H3K9me3 and Cell Transformation, Neoplastic |
| Necrosis Antibodies | >1 publications with H3K9me3 and Necrosis |
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