• Assay Conditions: 25mM Tris-HCl pH9,0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0,1 mg/mL gelatine, 200 μM de dATP, dGTP, dTTP, 100μM[α32-P]dCTP (0,05μCi/nmol) and 12,5 μg activated salmon sperm DNA.
• Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes:
– Horse-Power™ Taq Polymerase (5 U/μL)
– PCR Buffer (10x)
– 25 mM MgCl₂ (1.5 mL)
– TruePure™ dNTPs (2 mM each)
– GC Enhancer Solution (10x)

• Amplification of GC-rich DNA regions.

• Long-fragment PCR optimization.

• High-fidelity PCR assays.

• Cloning and sequencing of difficult targets.

• Each batch is tested for amplification performance, reproducibility, and enzyme compatibility.
• Undetected bacterial DNA (by PCR).
• Undetectable nucleases activity (endo-, exo, and ribonucleases).

• Add enhancer to your PCR mix according to recommended ratios.

• Optimize Mg²⁺ concentration for best performance.

• Avoid repeated freeze–thaw cycles; store aliquots when possible.

• Shipped in: Gel Pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in thi

GC PCR enhancer kit, PCR additive for GC-rich templates, GC-rich DNA amplification enhancer, PCR enhancer solution, High GC content PCR kit

Also known as:

• Spanish: Kit potenciador PCR para regiones ricas en GC, Aditivo PCR para ADN rico en GC, Solución potenciadora PCR
• French: Kit améliorateur PCR pour séquences riches en GC, Additif PCR ADN riche en GC, Solution amélioratrice PCR
• German: PCR-Enhancer-Kit für GC-reiche Sequenzen, PCR-Additiv für GC-reiche DNA, PCR-Verbesserungslösung
• Italian: Kit enhancer PCR per sequenze ricche in GC, Additivo PCR per DNA ricco in GC, Soluzione migliorativa PCR

Q1: What makes Horse-Power™ GC PCR Enhancer effective on GC-rich DNA?
It contains proprietary additives that destabilize secondary structures and enhance denaturation.

Q2: Is it compatible with Taq and high-fidelity polymerases?
Yes, it works with a wide range of DNA polymerases and PCR master mixes.

Q3: Does it affect downstream sequencing or cloning?
No, it does not interfere with subsequent enzymatic reactions or DNA manipulations.