RayBio® C-Series Human DNA Damage Response Phosphorylation Array 1 Kit. Detects 18 phosphorylated DNA damage response proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Human DNA Damage Response Phosphorylation Array C1
Human DNA Damage Response Phosphorylation Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 18 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, DNA Damage |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
Acetyl Histone H3 (K9), ATM (S1981), ATR (T1989), C-ABL (Y245), CDC2/CDK1 (Y15), CDC25a (S293), CHK1 (S296), CHK2 (T68), Cyclin B1 (S126), H2AX (S139), NBS1 (S343), P53 (S15), Cleaved PARP (D214/D215), PKMYT1 (T495), PLK1 (T210), RB1 (S811), RSK1 (S380), RSK2 (S386)
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Phosphorylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or plastic Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Typical Data
Figure 1 - HT-29 cells were either untreated (right panel) or treated (left panel). Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Note the strong signals of the Positive Control spots in the upper left and lower right corners. (See below for further details on the control spots.)
The signal intensity for each antigen-specific antibody spot is proportional to the relative concentration of the antigen in that sample. Comparison of signal intensities for individual antigen-specific antibody spots between and among array images can be used to determine relative differences in expression levels of each analyte sample-to-sample or group-to-group.
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.