RayBio® Human EGF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human EGF ELISA Kit
Human EGF ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 200 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | P01133 |
| Gene Id | 1950 |
| Gene Symbols | EGF |
| Protein Name / Synonyms | Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human EGF qIPCR ELISA Kit
As low as $639.00
RayPlex® Human EGF Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human EGF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified EGF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Development of Antiseptic and Epidermal Growth Factor Co-Loaded Thermoresponsive Composite Hydrogel for Wound Healing: Fabrication, Characterization, and In Vitro Functional Assessment
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (PBS release supernatants from PEHHPG hydrogel)
- Year:
- 2026
- Journal Name:
- Gels (Basel, Switzerland)
- PubMed ID:
- 42354437
Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatant from cultured cell-scaffold complex)
- Year:
- 2022
- Journal Name:
- Bioactive Materials
- PubMed ID:
- 35310356
Assessment Of Growth Factors Released From Partially Demineralized Tooth Matrix Based On Donor Age And Gender
- Species:
- Human
- Sample Type:
- Other / Unspecified (partially demineralized tooth matrix)
- Year:
- 2024
- Journal Name:
- African Journal of Biomedical Research
Comparing the release of growth factors when partially demineralized tooth matrix is mixed with platelet rich fibrin and injectable platelet rich fibrin: An in-vitro observational study
- Species:
- Human
- Sample Type:
- Other / Unspecified (PRF, i-PRF, platelet concentrates combined with PDTM)
- Year:
- 2024
- Journal Name:
- Journal of oral and maxillofacial pathology : JOMFP
- PubMed ID:
- 39670126
Gelatin-grafted tubular asymmetric scaffolds promote ureteral regeneration via activation of the integrin/Erk signaling pathway
- Species:
- Human
- Sample Type:
- human uroepithelial cells (HUC)
- Year:
- 2023
- Journal Name:
- Frontiers in bioengineering and biotechnology
Surface roughness modulates EGFR signaling and stemness of triple-negative breast cancer cells
- Species:
- Human
- Sample Type:
- MDA-MB-231 cell, MDA-MB-468 cell
- Year:
- 2023
- Journal Name:
- Frontiers in cell and developmental biology
Preparation of redispersible liposomal dry powder using an ultrasonic spray freeze-drying technique for transdermal delivery of human epithelial growth factor
- Year:
- 2023
- Journal Name:
- International Journal of Nanomedicine
Immediate Pre-Partum SARS-CoV-2 Status and Immune Profiling of Breastmilk: A Case-Control Study
- Species:
- Human
- Sample Type:
- Other Body Fluid (breastmilk)
- Year:
- 2021
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 34381462
Sonic hedgehog pathway activation is associated with cetuximab resistance and EPHB3 receptor induction in colorectal cancer
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (colorectal cancer cells)
- Year:
- 2019
- Journal Name:
- Theranostics
- PubMed ID:
- 31149041
3D co-culture model of endothelial colony-forming cells (ECFCs) reverses late passage adipose-derived stem cell senescence for wound healing
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture media from ASCs, ECFCs, ASC+ECFC co-cultures)
- Year:
- 2020
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 32795343
