Human IgA1 Mouse Monoclonal Antibody [Clone ID: NI 69-11 (A89-036)]
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SKU
AM20246AF-N
Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), Azide Free
Specifications
Product Data
Clone Name
NI 69-11 (A89-036)
Application
ELISA, IF, IP, WB
Recommended Dilution
To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/500 upwards Western blot: 1/3,000-1/50,000.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Highly purified monoclonal IgA1 isolated from pooled Human serum
Specificity
The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species. The reactivity of the antiserum is restricted to a subclass specific determinant on the CH2 domain of IgA1 as tested in Haemagglutination, Haemagglutination Inhibition, Direct Binding Enzyme Immunoassay, Competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
Buffer
State: Azide Free State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without preservatives and foreign proteins.
Presentation State
Azide Free
Reconstitution Method
Restore by adding 0.5 ml sterile distilled water.
Concentration
1.0 mg/ml
Conjugation
Unconjugated
Storage
Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
