RayBio® Human IL-10 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Human IL-10 ELISA Kit
Human IL-10 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 150 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P22301 |
| Gene Id | 3586 |
| Gene Symbols | IL10 |
| Protein Name / Synonyms | Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Target Background
IL-10 is an anti-inflammatory cytokine that plays an important role in the control of allergy and asthma. It can maintain the balance of the immune response while mitigating the damage to the host. IL-10 is synthesized by a variety of cell types, including B cells, monocytes, DCs, NK cells and T cells. It inhibits pro-inflammatory cytokine production and the activation of the TH1 and TH2 response. IL-10 gene polymorphisms are associated with diseases such as, asthma, psoriasis and inflammatory bowel disease.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IL-10 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-10 (IL10), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Exploration and analysis of methylglyoxal-driven chronic inflammation in polycystic ovary syndrome
- Year:
- 2026
- Journal Name:
- Journal of Advanced Research
- PubMed ID:
- 40865634
Enhanced sialylation of bone marrow mesenchymal stromal cells contributes to immune remodeling through macrophage polarization in multiple myeloma patients
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants from primary macrophages)
- Year:
- 2026
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 42712628
Targeting polymicrobial skin infections in atopic dermatitis: role of Pichia kudriavzevii postbiotics as innovative tools for human health
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture supernatants HaCaT)
- Year:
- 2026
- Journal Name:
- Frontiers in Microbiology
- PubMed ID:
- 42625856
Sleep disturbances, inflammation, and muscle function in older adults with sarcopenia
- Species:
- Human
- Sample Type:
- Whole Blood (older adults with sarcopenia)
- Year:
- 2026
- Journal Name:
- Sleep & breathing = Schlaf & Atmung
- PubMed ID:
- 42696225
Reduced IL-10 production by FoxP3+IL-10+ Treg cells is partially compensated by complosome-associated induction of regulatory FoxP3-IL-10+ T cells in allergic eosinophilic asthma
- Species:
- Human
- Sample Type:
- Plasma (allergic eosinophilic asthma)
- Year:
- 2026
- Journal Name:
- Clinical and Experimental Immunology
- PubMed ID:
- 41875927
Evaluating Pro- and Anti-inflammatory Biomarkers for Predicting Type 2 Diabetes Mellitus in the Geriatric Population and Correlation With Clinical and Biochemical Parameters
- Species:
- Human
- Sample Type:
- Serum (geriatric subjects, fasting)
- Year:
- 2025
- Journal Name:
- Curēus
- PubMed ID:
- 39991369
Exploring the Association Between Vitamin D and IL-10 in Allergic Parthenium Contact Dermatitis Patients
- Species:
- Human
- Sample Type:
- Plasma (allergic parthenium contact dermatitis patients)
- Year:
- 2025
- Journal Name:
- Curēus
- PubMed ID:
- 40630332
Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (SSc patient PBMCs treated with IL-40)
- Year:
- 2025
- Journal Name:
- Arthritis Research & Therapy
- PubMed ID:
- 40457473
Tumor-Associated CD19+CD39- B Regulatory Cells Deregulate Class-Switch Recombination to Suppress Antibody Responses
- Year:
- 2023
- Journal Name:
- Cancer Immunology Research
- PubMed ID:
- 36574614
Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cells
- Species:
- Human
- Sample Type:
- bone marrow-derived Mesenchymal stem cell
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy