RayBio® Human IL-18 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human IL-18 ELISA Kit
Human IL-18 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.5 pg/ml - 75 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | Q14116 |
| Gene Id | 3606 |
| Gene Symbols | IL18, IGIF, IL1F4 |
| Protein Name / Synonyms | Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines. Similar to IL-1b, it is synthesized as an inactive precursor which requires processing by caspase-1. However, unlike IL-1b, IL-18 is constitutively active in nearly all healthy cells. IL-18 binding protein (IL-18BP) has high affinity for IL-18, thus mediating the activity of IL-18. IL-18 forms a signaling complex with the IL-18Rα receptor and IL-18Rβ co-receptor to mediate NF?B pro-inflammatory signaling. IL-18BP, present in the extracellular compartment, binds mature IL-18 preventing binding to the IL-18 receptor. IL-18 has been implicated in conditions such as autoimmune disease, myocardial infarction, metabolic syndromes, inflammatory bowel disease and psoriasis.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IL-18 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-18 (IL18), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Cannabis use is associated with alterations in NLRP3 inflammasome related gene expression in monocyte-derived macrophages from people living with HIV
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture supernatants monocyte-derived macrophages)
- Year:
- 2025
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 41280902
Decreased expression of the NLRP6 inflammasome is associated with increased intestinal permeability and inflammation in obesity with type 2 diabetes
- Species:
- Human
- Sample Type:
- Serum (obesity with/without type 2 diabetes); Plasma
- Year:
- 2024
- Journal Name:
- Cellular and Molecular Life Sciences
- PubMed ID:
- 38315242
Platelet-Activating Factor Disrupts the Nasal Epithelial Barrier Independently of the Platelet-Activating Factor Receptor Pathway
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatant of hNECs)
- Year:
- 2025
- Journal Name:
- Allergy, asthma & immunology research
- PubMed ID:
- 40204506
Identifying a Role for the Sodium Hydrogen Exchanger Isoform 1 in Idiopathic Pulmonary Fibrosis: A Potential Strategy to Modulate Profibrotic Pathways
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants from cells cultured in 6-well plates)
- Year:
- 2025
- Journal Name:
- Biomedicines
- PubMed ID:
- 40299552
Serum cytokines profile changes in amyotrophic lateral sclerosis
- Species:
- Human
- Sample Type:
- Serum (ALS patients, validation cohort)
- Year:
- 2024
- Journal Name:
- Heliyon
- PubMed ID:
- 38596011
Urinary Cytokeratin 20 Predicts Severe Acute Kidney Injury and Major Adverse Kidney Events in Adults Undergoing Cardiac Surgery
- Year:
- 2025
- Journal Name:
- American Journal of Nephrology
- PubMed ID:
- 40319884
Interleukin-18 and Gelsolin Are Associated with Acute Kidney Disease after Cardiac Catheterization
- Species:
- Human
- Sample Type:
- urinary
- Year:
- 2023
- Journal Name:
- Biomolecules
Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation
- Species:
- Human
- Sample Type:
- Serum (female patients with clinical diagnosis of typical RTT and MECP2 mutation and healthy controls)
- Year:
- 2020
- Journal Name:
- Redox biology
- PubMed ID:
- 31606551
Human resistin is critical to activation of the NLRP3 inflammasome in macrophages
- Year:
- 2026
- Journal Name:
- PLoS ONE
- PubMed ID:
- 41961740
Activation of the Adipose Tissue NLRP3 Inflammasome Pathway in Cancer Cachexia
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture medium supernatant of adipose tissue explants)
- Year:
- 2021
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 34630405