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> Human Inflammation Array Q3

Human Inflammation Array Q3

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Referentie QAH-INF-3-1

Formaat : 8SampleKit

Merk : RayBiotech


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Human Inflammation Array Q3QAH-INF-3 was used to profile secreted inflammatory cytokines in conditioned media from senescent cells, revealing reduced SASP factor expression following METTL3 or METTL14 knockdown. Figure adapted from Liu et al., Nature Cell Biology, 2021.Figure adapted from Zhu et al., Research, 2025.Figure adapted from Mulfaul et al., Investigative Ophthalmology & Visual Science, 2024.Figure adapted from Pan et al., Molecular Cancer, 2022.Figure adapted from Nacarelli et al., Nature Cell Biology, 2019.Human Inflammation Array Q3Human Inflammation Array Q3

Human Inflammation Array Q3

Human Inflammation Array Q3

Multiplex ELISA array detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.
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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected40
Recommended ApplicationsMultiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaInflammation
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: 10563
  • Accession #: O43927
  • ELISA Kit: RayBio Human BLC ELISA
Eotaxin-1
(CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
  • ELISA Kit: RayBio Human Eotaxin-1 ELISA
Eotaxin-2
(MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: 6369
  • Accession #: O00175
  • ELISA Kit: RayBio Human Eotaxin-2 ELISA
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
  • ELISA Kit: RayBio Human G-CSF ELISA
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
  • ELISA Kit: RayBio Human GM-CSF ELISA
I-309
(TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
  • ELISA Kit: RayBio Human I-309 ELISA
ICAM-1
(CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
  • ELISA Kit: RayBio Human sICAM-1 ELISA
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
  • ELISA Kit: RayBio Human IFN-gamma ELISA
IL-1 alpha
(IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
  • ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
  • ELISA Kit: RayBio Human IL-1 beta ELISA
IL-1 ra
(IL-1 F3)
  • Gene Symbol: IL1RN
  • Gene ID: 3557
  • Accession #: P18510
  • ELISA Kit: RayBio Human IL-1 RA ELISA
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
  • ELISA Kit: RayBio Human IL-2 ELISA
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
  • ELISA Kit: RayBio Human IL-4 ELISA
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
  • ELISA Kit: RayBio Human IL-5 ELISA
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
  • ELISA Kit: RayBio Human IL-6 ELISA
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: 3570
  • Accession #: P08887
  • ELISA Kit: RayBio Human IL-6 sR ELISA
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
  • ELISA Kit: RayBio Human IL-7 ELISA
IL-8
(CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
  • ELISA Kit: RayBio Human IL-8 ELISA
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
  • ELISA Kit: RayBio Human IL-10 ELISA
IL-11
  • Gene Symbol: IL11
  • Gene ID: 3589
  • Accession #: P20809
  • ELISA Kit: RayBio Human IL-11 ELISA
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 3593
  • Accession #: P29460
  • ELISA Kit: RayBio Human IL-12 p40 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
  • ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
  • ELISA Kit: RayBio Human IL-13 ELISA
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
  • ELISA Kit: RayBio Human IL-15 ELISA
IL-16
  • Gene Symbol: IL16
  • Gene ID: 3603
  • Accession #: Q14005
  • ELISA Kit: RayBio Human IL-16 ELISA
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
MCP-1
(CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
  • ELISA Kit: RayBio Human MCP-1 ELISA
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
  • ELISA Kit: RayBio Human M-CSF ELISA
MIG
(CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
  • ELISA Kit: RayBio Human MIG ELISA
MIP-1 alpha
(CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
  • ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta
(CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
  • ELISA Kit: RayBio Human MIP-1 beta ELISA
MIP-1 delta
(CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
  • ELISA Kit: RayBio Human MIP-1 delta ELISA
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
  • ELISA Kit: RayBio Human PDGF-BB ELISA
RANTES
(CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
  • ELISA Kit: RayBio Human RANTES ELISA
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
  • ELISA Kit: RayBio Human TIMP-1 ELISA
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
  • ELISA Kit: RayBio Human TIMP-2 ELISA
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
  • ELISA Kit: RayBio Human TNF-alpha ELISA
TNF beta
(TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
  • ELISA Kit: RayBio Human TNF-beta ELISA
TNF RI
(TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 7132
  • Accession #: P19438
  • ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII
(TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: 7133
  • Accession #: P20333
  • ELISA Kit: RayBio Human sTNFRII ELISA

Typical Data

QAH-INF-3 was used to profile secreted inflammatory cytokines in conditioned media from senescent cells, revealing reduced SASP factor expression following METTL3 or METTL14 knockdown. Figure adapted from Liu et al., Nature Cell Biology, 2021.

QAH-INF-3 was used to profile inflammatory cytokines in astrocyte-conditioned media, revealing reduced IL-11 secretion following inflammatory stimulation and implicating IL-11 in astrocyte–microglia crosstalk during sepsis-associated encephalopathy. Figure adapted from Zhu et al., Research, 2025.

QAH-INF-3 was used to profile inflammatory cytokines in macular RPE/choroid tissue from AMD and control donors, with representative antibody array images showing elevated G-CSF expression in AMD samples. Figure adapted from Mulfaul et al., Investigative Ophthalmology & Visual Science, 2024.

QAH-INF-3 was used to profile inflammatory cytokines in conditioned media from anaplastic thyroid carcinoma cells and tumor cell–fibroblast co-cultures, identifying CREB3L1-dependent IL-1α secretion associated with stromal activation. Figure adapted from Pan et al., Molecular Cancer, 2022.

QAH-INF-3 was used to profile secreted inflammatory cytokines in conditioned media from senescent cells, with heat map analysis revealing suppression of the proinflammatory SASP following inhibition of the HMGA1–NAMPT–NAD⁺ axis. Figure adapted from Nacarelli et al., Nature Cell Biology, 2019.

Kit Components

  • Human Inflammation Array Q3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual

Additional Materials Required

  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Assay Procedure Summary

  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (QAH-INF-3.pdf)
SDS (QAH-INF-3.pdf)
GAL (GAL-QAH-INF-3.gal)
Excel (QAH-INF-3_Analysis_Tool.xls)

citations

  • A nested case-control study of air pollution and congenital heart defects via circulating proteins

    Species:
    Human
    Sample Type:
    Serum (maternal venous serum)
    Year:
    2026
    Journal Name:
    Nature Communications

  • Selenium-modified graphene oxide: A tri-dimensional study of its cytotoxicity and developmental effects

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (NHDF cells, GO-Se treated)
    Year:
    2026
    Journal Name:
    Materials Today Bio
    PubMed ID:
    41488428

  • Virus-immune signatures reveal distinct clinical phenotypes and predict prognosis in patients with Kaposi's sarcoma

    Species:
    Human
    Sample Type:
    Serum (12 KS patients, 10 healthy controls)
    Year:
    2026
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    42045930

  • Microbial Product Cocktails for Personalized Cancer Immunotherapy

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (unstimulated, MPC-stimulated PPS1-3 supernatants)
    Year:
    2025
    Journal Name:
    Nature Communications
    PubMed ID:
    41331244

  • Reduction of alternative polarization of macrophages by short-term activated hepatic stellate cell-derived small extracellular vesicles

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (THP-1 macrophage culture media)
    Year:
    2025
    Journal Name:
    Journal of Experimental & Clinical Cancer Research
    PubMed ID:
    40211350

  • Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton

    Year:
    2026
    Journal Name:
    Communications Biology
    PubMed ID:
    41781748

  • Senescent Fibroblasts Drive Melanoma Progression Through GCP-2 Induced CREB Phosphorylation Enhancing Glycolysis

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (conditioned media of young, senescent fibroblasts)
    Year:
    2025
    Journal Name:
    Aging Cell
    PubMed ID:
    41258756

  • Prenatal Infection by Respiratory Viruses Is Associated with Immunoinflammatory Responses in the Fetus

    Species:
    Human
    Sample Type:
    Whole Blood (maternal blood, cord blood)
    Year:
    2024
    Journal Name:
    American Journal of Respiratory and Critical Care Medicine
    PubMed ID:
    38051928

  • Dialog between mantle cell lymphoma cells and lymphoma-associated macrophages underlies ibrutinib resistance

    Year:
    2024
    Journal Name:
    Journal of advanced research
    PubMed ID:
    39168245

  • Development of machine-learning-driven signatures for diagnosing and monitoring therapeutic response in major depressive disorder using integrated immune cell profiles and plasma cytokines

    Species:
    Human
    Sample Type:
    Plasma (MDD patients and controls)
    Year:
    2024
    Journal Name:
    Theranostics
    PubMed ID:
    39629120

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